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Title: Determination of paraquat in blood and urine by liquid chromatography-electrospray-mass spectrometry
Authors: Proença, P. 
Vidinha, J.
Teixeira, H. 
Castanheira, F. 
Mendonça, M. C. 
Sousa, E.
Mustra, C.
Franco, J. M 
Corte-Real, F. 
Vieira, D. N. 
Issue Date: 2010
Publisher: Imprensa da Universidade de Coimbra; International Academy of Legal Medicine
Serial title, monograph or event: Acta Medicinae Legalis et Socialis
Place of publication or event: Coimbra
Abstract: Paraquat (PQ) is a toxic quaternary ammonium compound widely used in agriculture. Numerous cases of paraquat intoxication have been reported either accidentally or intentionally as suicidal attempts. A method for the determination of the herbicide PQ in blood and urine samples was developed using liquid chromatography-(electrospray ionization) mass spectrometry (LCESI-MS), following extraction with Oasis® WCX solid-phase cartridges. Chromatographic separation was achieved using an Atlantis® HILIC silica column, eluted isocratically with acetonitrile and ammonium formate (200mM) buffer, pH 3.8, at a 300 µL/min flow rate. Quantitation was achieved by the addition of ethyl paraquat as internal standard (IS). The compounds were detected monitoring two ions for PQ (m/z 185 and m/z 171) and m/z 213 for the IS. The method was applied to determine PQ in two cases: a non fatal case, a 42-year-old female with 0.13 µg/mL PQ concentration in blood and 6.29 µg/mL in urine and a lethal case, a 51-year-old male with 0.27 µg/ mL PQ concentration in blood. The authors developed a specific, sensitive and rapid assay for the identification and quantification of PQ, very important for monitoring suspected paraquat intoxications in hospitals and subsequently help in the treatment of these patients.
ISBN: 9789892601731
DOI: 10.14195/978-989-26-0173-1_56
Rights: openAccess
Appears in Collections:FMUC Medicina - Livros e Capítulos de Livros

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