Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/95043
DC FieldValueLanguage
dc.contributor.authorAguilar, Carmen-
dc.contributor.authorCosta, Susana-
dc.contributor.authorMaudet, Claire-
dc.contributor.authorVivek-Ananth, R P-
dc.contributor.authorZaldívar-López, Sara-
dc.contributor.authorGarrido, Juan J-
dc.contributor.authorSamal, Areejit-
dc.contributor.authorMano, Miguel-
dc.contributor.authorEulálio, Ana-
dc.date.accessioned2021-06-09T15:28:58Z-
dc.date.available2021-06-09T15:28:58Z-
dc.date.issued2021-
dc.identifier.issn2041-1723pt
dc.identifier.urihttp://hdl.handle.net/10316/95043-
dc.description.abstractCells infected with pathogens can contribute to clearing infections by releasing signals that instruct neighbouring cells to mount a pro-inflammatory cytokine response, or by other mechanisms that reduce bystander cells' susceptibility to infection. Here, we show the opposite effect: epithelial cells infected with Salmonella Typhimurium secrete host factors that facilitate the infection of bystander cells. We find that the endoplasmic reticulum stress response is activated in both infected and bystander cells, and this leads to activation of JNK pathway, downregulation of transcription factor E2F1, and consequent reprogramming of microRNA expression in a time-dependent manner. These changes are not elicited by infection with other bacterial pathogens, such as Shigella flexneri or Listeria monocytogenes. Remarkably, the protein HMGB1 present in the secretome of Salmonella-infected cells is responsible for the activation of the IRE1 branch of the endoplasmic reticulum stress response in non-infected, neighbouring cells. Furthermore, E2F1 downregulation and the associated microRNA alterations promote Salmonella replication within infected cells and prime bystander cells for more efficient infection.pt
dc.language.isoengpt
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/06572/2020/PT/PHDpt
dc.relationinfo:eu-repo/grantAgreement/POCI‐01‐0145‐FEDER-007440/PTpt
dc.relationinfo:eu-repo/grantAgreement/FCT/04539/2020/PTpt
dc.relationIF/01105/2015pt
dc.rightsopenAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt
dc.subjectBacterial pathogenesispt
dc.subjectCellular microbiologypt
dc.subjectmiRNAspt
dc.subjectPathogenspt
dc.titleReprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cellspt
dc.typearticle-
degois.publication.firstPage3392pt
degois.publication.issue1pt
degois.publication.titleNature Communicationspt
dc.relation.publisherversionhttps://www.nature.com/articles/s41467-021-23593-z#citeaspt
dc.peerreviewedyespt
dc.identifier.doi10.1038/s41467-021-23593-zpt
degois.publication.volume12pt
dc.date.embargo2021-01-01*
uc.date.periodoEmbargo0pt
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptUniversity of Coimbra-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-3020-5918-
crisitem.author.orcid0000-0003-1922-4824-
crisitem.author.orcid0000-0002-7355-0674-
crisitem.project.grantnoinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/06572/2020/PT/PHD-
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
s41467-021-23593-z.pdf2.29 MBAdobe PDFView/Open
Show simple item record

Google ScholarTM

Check

Altmetric

Altmetric


This item is licensed under a Creative Commons License Creative Commons