Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/93145
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dc.contributor.authorFerreira, Elisa-
dc.contributor.authorReal, Francisco Corte-
dc.contributor.authorMelo, Teresa Pinho e-
dc.contributor.authorMargalho, Cláudia-
dc.date.accessioned2021-02-17T11:55:41Z-
dc.date.available2021-02-17T11:55:41Z-
dc.date.issued2020-12-12-
dc.identifier.issn0146-4760pt
dc.identifier.issn1945-2403pt
dc.identifier.urihttp://hdl.handle.net/10316/93145-
dc.description.abstractOpioids are the drugs most commonly detected in overdose deaths and the second most consumed worldwide. An analytical methodology has been optimized and fully validated for the determination of codeine, morphine, 6-acetylmorphine, 6-acetylcodeine, oxycodone, oxymorphone and fentanyl in whole blood and pericardial fluid. The internal standards used were codeine-d3, morphine-d3, 6-acetylmorphine-d3 and fentanyl-d5. Before solid-phase extraction, volumes of 250 μL of blood and pericardial fluid were subjected to a protein precipitation (with 750 μL of ice-cold acetonitrile) and a microwave-induced oximation was performed using a solution of 1% aqueous hydroxylamine hydrochloride in phosphate-buffered saline (1:2, v/v). Finally, the dried extracts were further derivatized with a solution of n-methyl-n-(trimethylsilyl) trifluoroacetamide + 5% trimethylchlorosilane under microwave irradiation. The chromatographic analysis was carried out using gas chromatography-mass spectrometry operating in electron impact and selected ion monitoring mode. For all analytes, the method was linear between 5 and 1,000 ng/mL with determination coefficients (r2) >0.99. Depending on the analyte and matrix, the limit of detection varies between 3 and 4 ng/mL. Intra- and intermediate precision (<20%) and bias (±20%) were acceptable for all analytes in both matrices. The stability of the substances in the studied matrices was guaranteed, at least, 24 h in the autosampler, 4 h at room temperature and 30 days after three freeze/thaw cycles. This methodology was applied to real samples from the Laboratory of Chemistry and Forensic Toxicology, Centre Branch, of the National Institute of Legal Medicine and Forensic Sciences, Portugal.pt
dc.language.isoengpt
dc.rightsembargoedAccesspt
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt
dc.subject.meshAnalgesics, Opioidpt
dc.subject.meshDrug Overdosept
dc.subject.meshFentanylpt
dc.subject.meshHumanspt
dc.subject.meshOxycodonept
dc.subject.meshPericardial Fluidpt
dc.subject.meshSubstance Abuse Detectionpt
dc.subject.meshForensic Toxicologypt
dc.titleA Novel Bioanalytical Method for the Determination of Opioids in Blood and Pericardial Fluidpt
dc.typearticle-
degois.publication.firstPage754pt
degois.publication.lastPage768pt
degois.publication.issue8pt
degois.publication.titleJournal of Analytical Toxicologypt
dc.relation.publisherversionhttps://academic.oup.com/jat/article-abstract/44/8/754/5855107pt
dc.peerreviewedyespt
dc.identifier.doi10.1093/jat/bkaa064pt
degois.publication.volume44pt
dc.date.embargo2021-12-12*
uc.date.periodoEmbargo365pt
item.fulltextCom Texto completo-
item.grantfulltextembargo_20211212-
item.languageiso639-1en-
crisitem.author.deptFaculty of Pharmacy-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0003-3256-4954-
Appears in Collections:I&D CQC - Artigos em Revistas Internacionais
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This item is licensed under a Creative Commons License Creative Commons