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Title: Substrate Topography Modulates Cell Aging on a Progeria Cell Model
Authors: Pitrez, Patrícia R. 
Estronca, Luís Miguel Beicinha Branco 
Vazão, Helena 
Egesipe, Anne-Laure
Le Corf, Amélie
Navarro, Claire
Lévy, Nicolas 
De Sandre-Giovannoli, Annachiara
Nissan, Xavier
Ferreira, Lino 
Issue Date: 2018
Publisher: American Chemical Society
Project: This work was supported by funds from FEDER through COMPETE program and Fundação para a Ciência e a Tecnologia (FCT) (MITP-TB/ECE/0013/2013 to L.F.; SFRH/BD/71042/2010 to P.R.P.) as well as the European project ERAatUC (ref. 669088). 
Serial title, monograph or event: ACS Biomaterials Science & Engineering
Abstract: Aging is characterized by a progressive accumulation of cellular damage, which leads to impaired function. Little is known whether substrates can influence cell aging. This is of utmost importance in the development of medical devices that are in contact with human tissue for long periods of time. To address this question, we have used an accelerated aging cell model derived from Hutchinson-Gilford Progeria Syndrome (HGPS) induced pluripotent stem cells (iPSCs). Our results show that HGPS-iPSC smooth muscle cells (SMCs) have an increased aging profile in substrates with specific micropatterns than in flat ones. This is characterized by an up-regulation in the expression of progerin, β-galactosidase, annexin 3 and 5, and caspase 9. Signs of cell aging are also observed in SMCs without HGPS cultured in substrates with specific microtopographies. It is further showed that specific micropatterned substrates induce cell aging by triggering a DNA damage program likely by the disruption between cyto- and nucleoskeleton.
ISSN: 2373-9878
DOI: 10.1021/acsbiomaterials.8b00224
Rights: openAccess
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais

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