Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/92389
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dc.contributor.authorPraça, Catarina-
dc.contributor.authorRai, Akhilesh-
dc.contributor.authorSantos, Tiago-
dc.contributor.authorCristovão, Ana C.-
dc.contributor.authorPinho, Sonia L.-
dc.contributor.authorCecchelli, Romeo-
dc.contributor.authorDehouck, Marie-Pierre-
dc.contributor.authorBernardino, Liliana Inácio-
dc.contributor.authorFerreira, Lino S.-
dc.date.accessioned2020-12-31T15:31:33Z-
dc.date.available2020-12-31T15:31:33Z-
dc.date.issued2018-
dc.identifier.issn01683659pt
dc.identifier.urihttp://hdl.handle.net/10316/92389-
dc.description.abstractStimulation of adult neurogenesis by targeting the endogenous neural stem cells (NSCs), located in hippocampus and subventricular zone (SVZ), with nanoformulations has been proposed for brain repair in cases of neurodegenerative diseases. Unfortunately, it is relatively unknown the nanoformulation properties to facilitate their accumulation in the neurogenic niches after intravenous injection. Here, we have screened different gold-based formulations having variable morphology, surface chemistry and responsiveness to light for their capacity to cross the blood brain barrier (BBB) and accumulate preferentially in the neurogenic niches. Results obtained in a human in vitro BBB model showed that gold nanoparticles (Au NPs) and gold nanorods (Au NRs) conjugated with medium density of transferrin (Tf) peptides (i.e. between 169 and 230 peptides per NP) crossed more efficiently the BBB than the remaining formulations. This is due to a relatively lower avidity of these formulations to Tf receptor (TfR) and lower accumulation in the lysosomes, as compared to the other formulations. We further show that the near infrared light (NIR) irradiation of Au NRs, under a certain concentration and at specific cell culture time, lead to the opening of the BBB. Finally, we demonstrate that Au NRs conjugated with Tf administered intravenously in mice and activated by NIR had the highest accumulation in the neurogenic niches. Our results open the possibility of targeting more effectively the neurogenic niches by controlling the properties of the nanoformulations.pt
dc.language.isoengpt
dc.publisherElsevierpt
dc.rightsopenAccesspt
dc.subjectBlood-brain barrier; Brain targeting; NIR laser; Nanoparticles; Nanorods; Neurogenic nichespt
dc.subject.meshAnimalspt
dc.subject.meshBiological Transportpt
dc.subject.meshBlood-Brain Barrierpt
dc.subject.meshBrainpt
dc.subject.meshCell Linept
dc.subject.meshDrug Carrierspt
dc.subject.meshGoldpt
dc.subject.meshHumanspt
dc.subject.meshMetal Nanoparticlespt
dc.subject.meshMicept
dc.subject.meshNanoparticlespt
dc.subject.meshNanotubespt
dc.subject.meshNeurogenesispt
dc.subject.meshTransferrinpt
dc.titleA nanoformulation for the preferential accumulation in adult neurogenic nichespt
dc.typearticle-
degois.publication.firstPage57-72pt
degois.publication.lastPage72pt
degois.publication.titleJournal of Controlled Releasept
dc.peerreviewedyespt
dc.identifier.doi10.1016/j.jconrel.2018.06.013pt
degois.publication.volume284pt
dc.date.embargo2018-01-01*
uc.date.periodoEmbargo0pt
item.languageiso639-1en-
item.fulltextCom Texto completo-
item.grantfulltextopen-
crisitem.author.deptFaculty of Medicine-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0001-5352-5415-
crisitem.author.orcid0000-0001-8985-9302-
Appears in Collections:UC Bibliotecas - Artigos em Revistas Internacionais
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