Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/8240
Title: Simple measurement of gluconeogenesis by direct 2H NMR analysis of menthol glucuronide enrichment from 2H2O
Authors: Ribeiro, Angela 
Caldeira, M. Madalena 
Carvalheiro, Manuela 
Bastos, Margarida 
Baptista, Carla 
Fagulha, Ana 
Barros, Luisa 
Barosa, Cristina 
Jones, John G. 
Issue Date: 2005
Issue Date: 2005
Citation: Magnetic Resonance in Medicine. 54:2 (2005) 429-434
Abstract: The contribution of gluconeogenesis to fasting glucose production was determined by a simple measurement of urinary menthol glucuronide (MG) 2H enrichment from 2H2O. Following ingestion of 2H2O (0.5% body water) during an overnight fast and a pharmacological dose (400 mg) of a commercial peppermint oil preparation the next morning, 364 mumol MG was quantitatively recovered from a 2-h urine collection by ether extraction and a 125 mumol portion was directly analyzed by 2H NMR. The glucuronide 2H-signals were fully resolved and their relative intensities matched those of the monoacetone glucose derivative. The pharmacokinetics and yields of urinary MG after ingestion of 400 mg peppermint oil as either gelatin or enteric-coated capsules 1 h before breakfast were quantified in five healthy subjects. Gelatin capsules yielded 197 ± 81 mumol of MG from the initial 2-h urine collection while enteric-coated capsules gave 238 ± 84 mumol MG from the 2- to 4-h urine collection. Magn Reson Med 54:429-434, 2005. © 2005 Wiley-Liss, Inc.
URI: http://hdl.handle.net/10316/8240
DOI: 10.1002/mrm.20567
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

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