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Title: Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate
Authors: Carvalho, Rui A. 
Babcock, Evelyn E. 
Jeffrey, F. Mark H. 
Sherry, A. Dean
Malloy, Craig R.
Issue Date: 1999
Issue Date: 1999
Citation: Magnetic Resonance in Medicine. 42:1 (1999) 197-200
Abstract: Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl-CoA fractional enrichment (FC1 and FC3), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate. Magn Reson Med 42:197-200, 1999. © 1999 Wiley-Liss, Inc.
DOI: 10.1002/(SICI)1522-2594(199907)42:1<197
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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