Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/80403
Title: Chitosan-based dressings loaded with neurotensin--an efficient strategy to improve early diabetic wound healing
Authors: Moura, Liane I F 
Dias, Ana M A 
Leal, Ermelindo C 
Carvalho, Lina 
de Sousa, Hermínio C 
Carvalho, Eugénia 
Issue Date: Feb-2014
Abstract: One important complication of diabetes mellitus is chronic, non-healing diabetic foot ulcers (DFUs). This study aims to develop and use dressings based on chitosan derivatives for the sustained delivery of neurotensin (NT), a neuropeptide that acts as an inflammatory modulator in wound healing. Three different derivatives, namely N-carboxymethyl chitosan, 5-methyl pyrrolidinone chitosan (MPC) and N-succinyl chitosan, are presented as potential biomaterials for wound healing applications. Our results show that MPC has the best fluid handling capacity and delivery profile, also being non-toxic to Raw 264.7 and HaCaT cells. NT-loaded and non-loaded MPC dressings were applied to control/diabetic wounds to evaluate their in vitro/in vivo performance. The results show that the former induced more rapid healing (50% wound area reduction) in the early phases of wound healing in diabetic mice. A NT-loaded MPC foam also reduced expression of the inflammatory cytokine TNF-α (P<0.001) and decreased the amount of inflammatory infiltrate on day 3. On day 10 MMP-9 was reduced in diabetic skin (P<0.001), significantly increasing fibroblast migration and collagen (COL1A1, COL1A2 and COL3A1) expression and deposition. These results suggest that MPC-based dressings may work as an effective support for sustained NT release to reduce DFUs.
URI: http://hdl.handle.net/10316/80403
ISSN: 1878-7568
DOI: 10.1016/j.actbio.2013.09.040
Rights: openAccess
Appears in Collections:I&D CIEPQPF - Artigos em Revistas Internacionais

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