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Title: Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis
Authors: Oliveira, Helena 
Loureiro, João 
Filipe, Luísa 
Santos, Conceição 
Ramalho-Santos, João 
Sousa, Mário 
Pereira, Maria de Lourdes 
Keywords: Cadmium chloride; Flow cytometry; Lead chloride; Spermatogenesis; Testicular toxicity
Issue Date: 2006
Citation: Reproductive Toxicology. 22:3 (2006) 529-535
Abstract: Flow cytometry (FCM) is a powerful tool to evaluate cell DNA content and ploidy levels. We have assessed the accuracy of two protocols of nuclei isolation from paraffinized samples (P1 and P2) by comparing FCM results with those obtained using fresh material (F1-F3). After isolation, nuclei were stained with propidium iodide and quantitatively analysed by FCM for changes in germ cell ratios. Results obtained with Protocol P2 were similar to those obtained using the protocol that gave best results for fresh tissues (F2). Protocol P2 was then applied to paraffin embedded testicular samples from ICR-CD1 mice exposed to 1, 2 and 3 mg CdCl2/kg bw by single subcutaneous injection, and to 74 and 100 mg PbCl2/kg bw administered in four repeated doses. The highest doses of CdCl2 decreased the number of haploid (1C) cells and increased the number of diploid (2C), S phase and tetraploid (4C) cells. Treatment with PbCl2 did not induce significant changes in testicular cells subpopulations. These results support the usefulness of FCM in evaluating the effect of toxic substances on mouse spermatogenesis, using both fresh and paraffinized material.
DOI: 10.1016/j.reprotox.2006.03.009
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

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