Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5289
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dc.contributor.authorNunes, Patricia M.-
dc.contributor.authorCarvalho, Eugénia-
dc.contributor.authorJones, John G.-
dc.date.accessioned2008-09-01T15:39:40Z-
dc.date.available2008-09-01T15:39:40Z-
dc.date.issued2008en_US
dc.identifier.citationCarbohydrate Research. 343:9 (2008) 1486-1489en_US
dc.identifier.urihttp://hdl.handle.net/10316/5289-
dc.description.abstractGlycogen was quantified in rat adipocytes by isolation using conventional KOH digestion and ethanol precipitation, followed by hydrolysis and spectrophotometric assay of the glucose product. A concentration of 0.193 ± 0.020 [mu]mol glucosyl units/106 cells was recorded. When this procedure was modified by including a 4 h incubation with glucose oxidase prior to glycogen hydrolysis, the glycogen concentration was found to be 0.055 ± 0.008 [mu]mol glucosyl units/106 cells. Therefore in adipocytes, conventional glycogen assays give substantial overestimates due to incomplete removal of glucose during glycogen isolation. Contaminant glucose can be scavenged in a simple manner by incubation with glucose oxidase prior to glycogen hydrolysis.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6TFF-4S7906X-4/1/a08b7a48b48b25f3f821340d39b80c8fen_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectAdipocyteen_US
dc.subjectGlycogen extractionen_US
dc.subjectGlucose oxidaseen_US
dc.subjectAmyloglucosidaseen_US
dc.titleElimination of glucose contamination from adipocyte glycogen extractsen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.carres.2008.04.004-
item.grantfulltextopen-
item.languageiso639-1en-
item.fulltextCom Texto completo-
crisitem.author.deptCNC - Center for Neuroscience and Cell Biology-
crisitem.author.orcid0000-0002-3745-3885-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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