Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5003
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dc.contributor.authorGaweda, Sylwia-
dc.contributor.authorMorán, M. Carmen-
dc.contributor.authorPais, Alberto A. C. C.-
dc.contributor.authorDias, Rita S.-
dc.contributor.authorSchillén, Karin-
dc.contributor.authorLindman, Björn-
dc.contributor.authorMiguel, M. Graça-
dc.date.accessioned2008-09-01T15:02:15Z-
dc.date.available2008-09-01T15:02:15Z-
dc.date.issued2008en_US
dc.identifier.citationJournal of Colloid and Interface Science. 323:1 (2008) 75-83en_US
dc.identifier.urihttp://hdl.handle.net/10316/5003-
dc.description.abstractFluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WHR-4S7SV46-1/1/4fec487f09e6c653d0e22a0669f4e0d3en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDNA compactionen_US
dc.subjectCoexistenceen_US
dc.subjectIntermediate structuresen_US
dc.subjectFluorescence microscopyen_US
dc.subjectDynamic light scatteringen_US
dc.titleCationic agents for DNA compactionen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.jcis.2008.04.006-
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.deptFaculty of Sciences and Technology-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.parentdeptUniversity of Coimbra-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.researchunitCQC - Coimbra Chemistry Centre-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.parentresearchunitFaculty of Sciences and Technology-
crisitem.author.orcid0000-0002-6725-6460-
crisitem.author.orcid0000-0002-7493-9112-
crisitem.author.orcid0000-0002-1601-5501-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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