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dc.contributor.authorGaweda, Sylwia-
dc.contributor.authorMorán, M. Carmen-
dc.contributor.authorPais, Alberto A. C. C.-
dc.contributor.authorDias, Rita S.-
dc.contributor.authorSchillén, Karin-
dc.contributor.authorLindman, Björn-
dc.contributor.authorMiguel, M. Graça-
dc.identifier.citationJournal of Colloid and Interface Science. 323:1 (2008) 75-83en_US
dc.description.abstractFluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.en_US
dc.subjectDNA compactionen_US
dc.subjectIntermediate structuresen_US
dc.subjectFluorescence microscopyen_US
dc.subjectDynamic light scatteringen_US
dc.titleCationic agents for DNA compactionen_US
item.fulltextCom Texto completo-
item.grantfulltextopen- de Ciências e Tecnologia, Universidade de Coimbra- de Ciências e Tecnologia, Universidade de Coimbra- de Ciências e Tecnologia, Universidade de Coimbra- de Coimbra- de Coimbra- de Coimbra- Chemistry Center- Chemistry Center- Chemistry Center-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
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