Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/5003
DC FieldValueLanguage
dc.contributor.authorGaweda, Sylwia-
dc.contributor.authorMorán, M. Carmen-
dc.contributor.authorPais, Alberto A. C. C.-
dc.contributor.authorDias, Rita S.-
dc.contributor.authorSchillén, Karin-
dc.contributor.authorLindman, Björn-
dc.contributor.authorMiguel, M. Graça-
dc.date.accessioned2008-09-01T15:02:15Z-
dc.date.available2008-09-01T15:02:15Z-
dc.date.issued2008en_US
dc.identifier.citationJournal of Colloid and Interface Science. 323:1 (2008) 75-83en_US
dc.identifier.urihttp://hdl.handle.net/10316/5003-
dc.description.abstractFluorescence microscopy was used to investigate the conformational changes of individual T4 DNA molecules induced by different compacting agents, namely the cationic surfactants, cetyltrimethylammonium bromide (CTAB) and chloride (CTAC), iron(III), lysozyme, and protamine sulfate. A protocol for establishing size estimates is suggested to obtain reproducible results. Observations show that in the presence of lysozyme and protamine sulfate, DNA molecules exhibit a conformational change from an elongated coil structure to compact globules, usually interpreted as a first-order transition. The maximum degree of compaction that is attained when iron(III) or CTAB (CTAC) are used as compacting agents is considerably smaller, and intermediate structures (less elongated coils) are visible even for high concentrations of these agents. Dynamic light scattering experiments were carried out, for some of the systems, to assess the reliability of size estimates from fluorescence microscopy.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6WHR-4S7SV46-1/1/4fec487f09e6c653d0e22a0669f4e0d3en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectDNA compactionen_US
dc.subjectCoexistenceen_US
dc.subjectIntermediate structuresen_US
dc.subjectFluorescence microscopyen_US
dc.subjectDynamic light scatteringen_US
dc.titleCationic agents for DNA compactionen_US
dc.typearticleen_US
uc.controloAutoridadeSim-
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.deptFaculdade de Ciências e Tecnologia, Universidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.parentdeptUniversidade de Coimbra-
crisitem.author.researchunitCoimbra Chemistry Center-
crisitem.author.researchunitCoimbra Chemistry Center-
crisitem.author.researchunitCoimbra Chemistry Center-
crisitem.author.orcid0000-0002-6725-6460-
crisitem.author.orcid0000-0002-7493-9112-
crisitem.author.orcid0000-0002-1601-5501-
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais
Files in This Item:
File Description SizeFormat
file6aec61166c604e51bd9b0196332329ab.pdf425.49 kBAdobe PDFView/Open
Show simple item record

Page view(s)

165
checked on Aug 12, 2020

Download(s)

95
checked on Aug 12, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.