Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/4790
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dc.contributor.authorCunha, Rodrigo A.-
dc.contributor.authorRibeiro, J. A.-
dc.contributor.authorMalva, João O.-
dc.date.accessioned2008-09-01T14:14:31Z-
dc.date.available2008-09-01T14:14:31Z-
dc.date.issued2004en_US
dc.identifier.citationNeurochemistry International. 44:5 (2004) 371-379en_US
dc.identifier.urihttp://hdl.handle.net/10316/4790-
dc.description.abstractKainate receptors are ionotropic glutamate receptors located postsynaptically, mediating frequency-dependent transmission, and presynaptically, modulating transmitter release. In contrast to the excitatory postsynaptic kainate receptors, presynaptic kainate receptor can also be inhibitory and their effects may involve a metabotropic action. Arachidonic acid (AA) modulates most ionotropic receptors, in particular postsynaptic kainate receptor-mediated currents. To further explore differences between pre- and postsynaptic kainate receptors, we tested if presynaptic kainate receptors are affected by AA. Kainate (0.3-3 [mu]M) and the kainate receptor agonist, domoate (60-300 nM), inhibited by 19-54% the field excitatory postsynaptic potential (fEPSP) slope in rat CA1 hippocampus, and increased by 12-32% paired-pulse facilitation (PPF). AA (10 [mu]M) attenuated by 37-72% and by 62-66% the domoate (60-300 nM)-induced fEPSP inhibition and paired-pulse facilitation increase, respectively. This inhibition by AA was unaffected by cyclo- and lipo-oxygenase inhibitors, indomethacin (20 [mu]M) and nordihydroguaiaretic acid (NDGA, 50 [mu]M) or by the free radical scavenger, N-acetyl--cysteine (0.5 mM). The K+ (20 mM)-evoked release of [3H]glutamate from superfused hippocampal synaptosomes was inhibited by 18-39% by domoate (1-10 [mu]M), an effect attenuated by 35-63% by AA (10 [mu]M). Finally, the KD (40-55 nM) of the kainate receptor agonist [3H]-(2S,4R)-4-methylglutamate ([3H]MGA) (0.3-120 nM) binding to hippocampal synaptosomal membranes was increased by 151-329% by AA (1-10 [mu]M). These results indicate that AA directly inhibits presynaptic kainate receptor controlling glutamate release in the CA1 area of the rat hippocampus.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6T0B-49JHH14-1/1/306d6b018d3a5d33b6a0973186ea4ed9en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.subjectKainateen_US
dc.subjectArachidonic aciden_US
dc.subjectHippocampusen_US
dc.subjectSynaptic transmissionen_US
dc.subjectNerve terminalsen_US
dc.subjectGlutamate releaseen_US
dc.subjectBindingen_US
dc.titlePresynaptic kainate receptors modulating glutamatergic transmission in the rat hippocampus are inhibited by arachidonic aciden_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.grantfulltextopen-
item.languageiso639-1en-
crisitem.author.researchunitCNC.IBILI-
crisitem.author.orcid0000-0002-5438-4447-
Appears in Collections:FMUC Medicina - Artigos em Revistas Internacionais
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