Please use this identifier to cite or link to this item:
Title: Determination of Metabolic Viability and Cell Mass Using a Tandem Resazurin/Sulforhodamine B Assay
Authors: Silva, Filomena S. G. 
Starostina, Irina G. 
Ivanova, Vilena V. 
Rizvanov, Albert A. 
Oliveira, Paulo J. 
Pereira, Susana P. 
Keywords: Animals; Biological Assay; Cell Line; Cell Size; Cell Survival; Colorimetry; Coloring Agents; Cytological Techniques; Fibroblasts; Humans; Indicators and Reagents; Mice; Oxazines; Reproducibility of Results; Rhodamines; Toxicology; Xanthenes
Issue Date: 4-May-2016
Serial title, monograph or event: Current protocols in toxicology
Volume: 68
Abstract: The identification of rapid, reliable, and highly reproducible biological assays that can be standardized and routinely used in preclinical tests constitutes a promising approach to reducing drug discovery costs and time. This unit details a tandem, rapid, and reliable cell viability method for preliminary screening of chemical compounds. This assay measures metabolic activity and cell mass in the same cell sample using a dual resazurin/sulforhodamine B assay, eliminating the variation associated with cell seeding and excessive manipulations in assays that test different cell samples across plates. The procedure also reduces the amount of cells, test compound, and reagents required, as well as the time expended in conventional tests, thus resulting in a more confident prediction of toxic thresholds for the tested compounds. © 2016 by John Wiley & Sons, Inc.
DOI: 10.1002/cptx.1
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

Show full item record


checked on Feb 18, 2020

Page view(s) 50

checked on Sep 13, 2022

Download(s) 50

checked on Sep 13, 2022

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.