Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/45036
Title: Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells
Authors: Medeiros, P. S. C. 
Batista de Carvalho, A. L. M. 
Ruano, C. 
Otero, J. C. 
Marques, M. P. M. 
Issue Date: 15-Jun-2016
Serial title, monograph or event: Food & function
Volume: 7
Issue: 6
Abstract: Breast cancer is the second most common type of cancer worldwide and the most frequent among women, being the fifth cause of death from neoplastic disease. Since this is an oxidative-stress related neoplasia, it is largely preventable. A dietary isoflavone abundant in soybean - daidzein - is currently being investigated owing to its chemopreventive and/or chemotherapeutic properties towards the human MDA-MB-231 (metastatic, estrogen-unresponsive) and MCF-7 (estrogen-responsive) breast cancer cell lines. Biological assays for evaluation of antitumour and anti-invasive activities were combined with state-of-the-art vibrational microspectroscopy techniques. At 50 and 100 μM concentrations and 48 h incubation time, daidzein was found to induce a marked decrease in cell viability (ca. 50%) for MDA-MB-231 and MCF-7 cells (respectively ca. 50% and 42%) and 40% inhibition of cell migration. MicroRaman analysis of fixed cells upon exposure to this isoflavone unveiled its metabolic impact on both cell lines. Multivariate data analysis (unsupervised PCA) led to a clear discrimination between the control and DAID-exposed cells, with distinctive effects on their biochemical profile, particularly regarding DNA, lipids and protein components, in a cell-dependent way. This is the first reported study on the impact of dietary antioxidants on cancer cells by microRaman techniques.
URI: http://hdl.handle.net/10316/45036
DOI: 10.1039/C6FO00209A
10.1039/c6fo00209a
Rights: openAccess
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
FoodFunct_16.pdf1.24 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

5
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations 10

9
checked on Oct 2, 2021

Page view(s)

216
checked on Oct 8, 2021

Download(s)

245
checked on Oct 8, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.