Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/3903
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dc.contributor.authorJones, John G.-
dc.contributor.authorCarvalho, Rui A.-
dc.contributor.authorFranco, Byron-
dc.contributor.authorSherry, A. Dean-
dc.contributor.authorMalloy, Craig R.-
dc.date.accessioned2008-08-29T15:35:54Z-
dc.date.available2008-08-29T15:35:54Z-
dc.date.issued1998en_US
dc.identifier.citationAnalytical Biochemistry. 263:1 (1998) 39-45en_US
dc.identifier.urihttp://hdl.handle.net/10316/3903-
dc.description.abstract13C and1H NMR spectroscopy of plasma glucose was used to resolve the isotopomer contributions from tracer levels of [1,6-13C2]glucose, a novel tracer of glucose carbon skeleton turnover, and [U-13C]propionate, a tracer of hepatic citric acid cycle metabolism. This allowed simultaneous measurements of hepatic glucose production and citric acid cycle fluxes from the NMR analysis of a single plasma glucose sample in fasted animals. Glucose carbon skeleton turnover, as reported by the dilution of [1,6-13C2]glucose, was 56 ± 2 [mu]mol/kg/min in the presence of labeling from [U-13C]propionate and 53 ± 4 [mu]mol/kg/min in its absence. Therefore, as expected, the labeling contributions from [U-13C]propionate metabolism did not have a significant effect on the measurement of glucose turnover. For the group infused with both tracers, citric acid cycle flux estimates from the analysis of glucose C2 isotopomer ratios were consistent with those from our recent experiments where only [U-13C]propionate was infused, verifying that the presence of [1,6-13C2]glucose did not interfere with these measurements. This integrated analysis of hepatic glucose output and citric acid cycle fluxes from plasma glucose isotopomers yielded a noninvasive estimate of hepatic citrate synthase flux of 74 ± 12 [mu]mol/kg/min for 24-h fasted rats.en_US
dc.description.urihttp://www.sciencedirect.com/science/article/B6W9V-45KNBC6-6/1/0b98622e9538e88b1c65cd4c5b78efc6en_US
dc.format.mimetypeaplication/PDFen
dc.language.isoengeng
dc.rightsopenAccesseng
dc.titleMeasurement of Hepatic Glucose Output, Krebs Cycle, and Gluconeogenic Fluxes by NMR Analysis of a Single Plasma Glucose Sampleen_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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