Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27908
Title: The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy
Authors: Krzykawska-Serda, Martyna 
Dąbrowski, Janusz M. 
Arnaut, Luis G. 
Szczygieł, Małgorzata 
Urbańska, Krystyna 
Stochel, Grażyna 
Elas, Martyna 
Keywords: Oxymetry; Blood flow; Vascular-targeted PDT; Bacteriochlorins; Hydroxyl radical; Singlet oxygen; Superoxide; Phototoxicity; Photodynamic therapy; Free radicals
Issue Date: Aug-2014
Publisher: Elsevier
Citation: KRZYKAWSKA-SERDA, Martyna [et. al] - The role of strong hypoxia in tumors after treatment in the outcome of bacteriochlorin-based photodynamic therapy. "Free Radical Biology and Medicine". ISSN 0891-5849. Vol. 73 (2014) p. 239–251
Serial title, monograph or event: Free Radical Biology and Medicine
Volume: 73
Abstract: Blood flow and pO2 changes after vascular-targeted photodynamic therapy (V-PDT) or cellular-targeted PDT (C-PDT) using 5,10,15,20-tetrakis(2,6-difluoro-3-N-methylsulfamoylphenyl) bacteriochlorin (F2BMet) as photosensitizer were investigated in DBA/2 mice with S91 Cloudman mouse melanoma, and correlated with long-term tumor responses. F2BMet generates both singlet oxygen and hydroxyl radicals under near-infrared radiation, which consume oxygen. Partial oxygen pressure was lowered in PDT-treated tumors and this was ascribed both to oxygen consumption during PDT and to fluctuations in oxygen transport after PDT. Similarly, microcirculatory blood flow changed as a result of the disruption of blood vessels by the treatment. A novel noninvasive approach combining electron paramagnetic resonance oximetry and laser Doppler blood perfusion measurements allowed longitudinal monitoring of hypoxia and vascular function changes in the same animals, after PDT. C-PDT induced parallel changes in tumor pO2 and blood flow, i.e., an initial decrease immediately after treatment, followed by a slow increase. In contrast, V-PDT led to a strong and persistent depletion of pO2, although the microcirculatory blood flow increased. Strong hypoxia after V-PDT led to a slight increase in VEGF level 24 h after treatment. C-PDT caused a ca. 5-day delay in tumor growth, whereas V-PDT was much more efficient and led to tumor growth inhibition in 90% of animals. The tumors of 44% of mice treated with V-PDT regressed completely and did not reappear for over 1 year. In conclusion, mild and transient hypoxia after C-PDT led to intense pO2 compensatory effects and modest tumor inhibition, but strong and persistent local hypoxia after V-PDT caused tumor growth inhibition.
URI: http://hdl.handle.net/10316/27908
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2014.05.003
Rights: openAccess
Appears in Collections:FCTUC Química - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
The role of strong hypoxia in tumors after treatment in the outcome.pdf4.3 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

37
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations 5

56
checked on Dec 2, 2021

Page view(s) 50

517
checked on Dec 8, 2021

Download(s) 50

457
checked on Dec 8, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.