Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/27674
Title: Role of Endothelial Progenitor Cells and Inflammatory Cytokines in Healing of Diabetic Foot Ulcers
Authors: Tecilazich, Francesco 
Dinh, Thanh 
Pradhan-Nabzdyk, Leena 
Leal, Ermelindo 
Tellechea, Ana 
Kafanas, Antonios 
Gnardellis, Charalambos 
Magargee, Mary L. 
Dejam, Andre 
Toxavidis, Vasilis 
Tigges, John C. 
Carvalho, Eugénia 
Lyons, Thomas E. 
Veves, Aristidis 
Issue Date: 16-Dec-2013
Publisher: PLOS
Citation: TECILAZICH, Francesco [et. al] - Role of Endothelial Progenitor Cells and Inflammatory Cytokines in Healing of Diabetic Foot Ulcers. "PLOS one". ISSN 1932-6203. Vol. 8 Nº. 12 (2013) p. e83314
Serial title, monograph or event: PLOS one
Volume: 8
Issue: 12
Abstract: Background: To evaluate changes in endothelial progenitor cells (EPCs) and cytokines in patients with diabetic foot ulceration (DFU) in association with wound healing. Methods: We studied healthy subjects, diabetic patients not at risk of DFU, at risk of DFU and with active DFU. We prospectively followed the DFU patients over a 12-week period. We also investigated similar changes in diabetic rabbit and mouse models of wound healing. Results: All EPC phenotypes except the kinase insert domain receptor (KDR)+CD133+ were reduced in the at risk and the DFU groups compared to the controls. There were no major EPC differences between the control and not at risk group, and between the at risk and DFU groups. Serum stromal-cell derived factor-1 (SDF-1) and stem cell factor (SCF) were increased in DFU patients. DFU patients who healed their ulcers had lower CD34+KDR+ count at visits 3 and 4, serum c-reactive protein (CRP) and granulocyte-macrophage colony-stimulating factor (GM-CSF) at visit 1, interleukin-1 (IL-1) at visits 1 and 4. EPCs tended to be higher in both diabetic animal models when compared to their non-diabetic counterparts both before and ten days after wounding. Conclusions: Uncomplicated diabetes does not affect EPCs. EPCs are reduced in patients at risk or with DFU while complete wound healing is associated with CD34+KDR+ reduction, suggesting possible increased homing. Low baseline CRP, IL-1α and GM-CSF serum levels were associated with complete wound healing and may potentially serve as prognostic markers of DFU healing. No animal model alone is representative of the human condition, indicating the need for multiple experimental models.
URI: http://hdl.handle.net/10316/27674
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0083314
Rights: openAccess
Appears in Collections:I&D CNC - Artigos em Revistas Internacionais

Files in This Item:
File Description SizeFormat
Role of Endothelial Progenitor.pdf1.6 MBAdobe PDFView/Open
Show full item record

SCOPUSTM   
Citations

33
checked on Feb 18, 2020

WEB OF SCIENCETM
Citations 5

44
checked on Nov 2, 2021

Page view(s) 10

954
checked on Nov 26, 2021

Download(s) 20

809
checked on Nov 26, 2021

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.