Please use this identifier to cite or link to this item: http://hdl.handle.net/10316/12641
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dc.contributor.authorCarvalho, Rui A.-
dc.contributor.authorRodrigues, Tiago B.-
dc.contributor.authorZhao, Piyu-
dc.contributor.authorJeffrey, F. Mark H.-
dc.contributor.authorMalloy, Craig R.-
dc.contributor.authorSherry, A. Dean-
dc.date.accessioned2010-03-02T12:22:02Z-
dc.date.available2010-03-02T12:22:02Z-
dc.date.issued2004-08-
dc.identifier.citationAmerican Journal of Physiology - Heart and Circulatory Physiology. 287 (2004) H889-H895en_US
dc.identifier.issn0363-6135-
dc.identifier.urihttp://hdl.handle.net/10316/12641-
dc.description.abstractRat hearts were perfused with mixtures of [3-(13)C]pyruvate and [3-(13)C]lactate (to alter cytosolic redox) at low (0.5 mM) or high (2.5 mM) Ca(2+) concentrations to alter contractility. Hearts were frozen at various times after exposure to these substrates, were extracted, and were then analyzed by (13)C NMR spectroscopy. The time-dependent multiplets observed in the (13)C NMR resonances of glutamate in all hearts and in malate and aspartate in hearts perfused with high-pyruvate/low-lactate concentrations were analyzed using a kinetic model of the tricarboxylic acid (TCA) cycle. The analysis showed that TCA cycle flux (V(TCA)) and exchange flux (V(X)) that involved cycle intermediates were both sensitive to cell redox and altered Ca(2+) concentration, and the ratio of these fluxes (V(X)/V(TCA)) varied >10-folden_US
dc.language.isoengen_US
dc.publisherThe American Physiological Societyen_US
dc.rightsopenAccessen_US
dc.titleA (13)C isotopomer kinetic analysis of cardiac metabolism: influence of altered cytosolic redox and [Ca(2+)](o)en_US
dc.typearticleen_US
item.fulltextCom Texto completo-
item.languageiso639-1en-
item.grantfulltextopen-
Appears in Collections:FCTUC Ciências da Vida - Artigos em Revistas Internacionais
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